Optimisation de la méthode d'extraction et de stabilisation de la phycocyanine utilisant la méthodologie de surface de réponse "RSM"

Authors

  • Khaoula BEN SLIMANE Université de Carthage. Institut National des Sciences et Technologies de la Mer. Laboratoire de Biodiversité et de Biotechnologie Marines, Vitalight LAB SA – Sidi Thabet. Tunisie
  • Saloua SADOK Université de Carthage. Institut National des Sciences et Technologies de la Mer. Laboratoire de Biodiversité et de Biotechnologie Marines. Tunisie
  • Hatem BEN OUADA Université de Carthage. Institut National des Sciences et Technologies de la Mer. Laboratoire de Biodiversité et de Biotechnologie Marines. Tunisie
  • Ilyes GOUJA Université de Carthage. Institut National des Sciences et Technologies de la Mer. Vitalight LAB SA – Sidi Thabet. Tunisie

Keywords:

extraction, phycocyanin, stabilization, Spirulina platensis

Abstract

A new method based on the response surface methodology (RSM) was developed for optimization and modeling of extraction and stabilization of phycocyanin which is a majority blue protein pigment of Spirulina platensis. The RSM method, based on the Composite Plan (CD) of the second degree allowes to study three variables at three levels to obtain the optimal combination of extraction and stabilization of phycocyanin. According to the response of 3D surface, the optimum extraction of phycocyanin is reached in the special conditions: pH = 5, temperature = 5 °C and compression ratio = 60% stabilizer / 40% solvent with an experimental value 80.36 mg /g DM.

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Published

2015-12-21

How to Cite

1.
BEN SLIMANE K, SADOK S, BEN OUADA H, GOUJA I. Optimisation de la méthode d’extraction et de stabilisation de la phycocyanine utilisant la méthodologie de surface de réponse "RSM". Bull. Inst. Natl. Sci. Technol. Mer [Internet]. 2015Dec.21 [cited 2024Apr.24];42:13-5. Available from: https://www.instm-bulletin.tn/index.php/bulletin/article/view/362

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ARK

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