INSTM Bulletin : Marine and Freshwater Sciences <p>The<strong> INSTM Bulletin: Marine and Freshwater Sciences </strong><span style="font-weight: 400;">is an annual Bulletin successor of the INSTOP Bulletin and the SOS Bulletin created in 1924. It is a peer-reviewed open access bulletin which publishes original papers related to </span><strong>Marine</strong><span style="font-weight: 400;"> and </span><strong>Freshwater Sciences</strong><span style="font-weight: 400;"> research</span><span style="font-weight: 400;"> including various fields such as</span> <strong>Oceanography, Biology</strong><span style="font-weight: 400;">, </span><strong>Ecology</strong><span style="font-weight: 400;">, </span><strong>Biodiversity, Biotechnology, Geology, Pollution</strong><span style="font-weight: 400;">, </span><strong>Toxicology, Fishery, Seafood and Aquaculture</strong><span style="font-weight: 400;">.</span></p> <p><span style="font-weight: 400;">The key criteria are that the work clearly demonstrates its novelty, its importance to a particular field as well as its interest to the wide scientific community. Reviews on current topics are welcome. Selected papers or extended abstracts from symposia or workshops may also be considered. Short notes about new records of marine species are accepted.</span></p> <p><span style="font-weight: 400;">The current <a href="">Bulletin’s archive</a> contains several hundred articles </span><span style="font-weight: 400;">covered in Google Scholar and indexed and abstracted in the</span> <strong>Aquatic Sciences and Fisheries Abstracts (ASFA)</strong> <strong>database</strong><span style="font-weight: 400;">. <a href="">See More</a></span></p> INSTM - Institut National des Sciences et Technologies de la Mer en-US INSTM Bulletin : Marine and Freshwater Sciences 0330-0080 Resistance profiling, evolution of biofilm formation and genetic diversity of Vibrio alginolyticus isolated from aquaculture systems <p>This study analyzes 26 presumptive <em>Vibrio</em><em> alginolyticus</em> strains extracted from two fish farms (Khenis and Hergla, East Tunisia) that raised sea bream (<em>Sparus aurata</em>) and sea bass (<em>Dicentrarchus labrax</em>). In addition, from a shellfish farm located in Menzel Jmil, North Tunisia that raises mussels (<em>Mytilus edulis</em>) and oysters (<em>Crassostrea gigas</em>).</p> <p>We have evaluated (i) the discriminatory power of PCR-RFLP for identification of closely related strains, (ii) the antibiotic resistance of the <em>V. alginolyticus</em> strains and (iii) their ability to form a biofilm in aquaculture farm.</p> <p>Results showed that there is great heterogeneity in the diversity observed via the PCR-RFLP method related to the number of 20 genotypes generated by the two enzymes <em>Sdu</em>I and <em>Faq</em>I tested. The heterogeneity was observed in both fish (aquaculture farm) and bivalve (shellfish farm) origins, as well as in the same sample.</p> <p>For antibiotic resistance, all isolates present a high resistance to ampicillin, erythromycin, cefotaxime, kanamycin and doxycycline. The resistance is displayed by 15 distinct profiles.</p> <p>The Multiple Antibiotic resistance (MAR) index was ranged from 0.55 to 0.80 for the isolates from the aquaculture farm of Hergla followed by the index aquaculture farm of Khenis (0.55 to 0.75). While for the shellfish farm of Menzel Jmil the index ranges from 0.50 to 0.65. This finding indicated high-risk sources of antibiotic contamination in the three locations. Isolates from aquaculture systems with strong biofilm formation have been found to be resistant to antibiotics, which may allow them to survive longer in these environments.</p> <p>Present findings could be relevant in aquaculture systems and underscore the importance of the linkage between adhesion, antibiotic susceptibility, and genetic diversity of these pathogenic bacteria to avoid fish and shellfish diseases. The results will provide helpful guidance on how to use antibiotics to control <em>Vibrio alginolyticus</em> diseases in aquaculture to be healthy.</p> Rim LAJNEF Miguel Angel MORIÑIGO Abdennaceur HASSEN Lotfi BEN ABDALLAH Copyright (c) 2024 Rim LAJNEF, Miguel Angel MORIÑIGO, Abdennaceur HASSEN, Lotfi BEN ABDALLAH 2024-05-17 2024-05-17 49 2 20 10.80367/instmbulletin.v49.1674 Age and growth of Carcinus aestuarii Nardo,1847 (Portunidae) and Maja squinado Herbst, 1788 (Majidae) in the Gulf of Gabes, Southeastern Tunisia, Central Mediterranean <p>The Mediterranean green crab, <em>Carcinus aestuarii</em> Nardo, 1847 (Crustacea, Brachyura, Portunidae), and the spider crab <em>Maja squinado</em> (Herbst) are common inhabitant of the shallow waters in the Gulf of Gabes (southern Tunisia, central Mediterranean).&nbsp;</p> <p>The Von Bertalanffy growth parameters CW∞/CL∞, k and t<sub>0</sub> were estimated on the basis of Length-frequency modal progression analysis method. The values of CW∞ (mm), K (year<sup>-1</sup>) and t<sub>0</sub> (year) would be, respectively, 95.61, 0.84 and -0.104 for males; 73.92, 1.11 and -0.149 for females in <em>Carcinus aestuarii</em> and, CL∞ (mm), K (year<sup>-1</sup>) and t0 (year) would be, respectively, 172.46, 0.68 and -0.147 for males; 147.05, 0.6 and -0.175 for females in <em>Maja squinado</em>.</p> <p>These results were obtained, as the first attempt to study age and growth of these two crustaceans which will be of a great use for their eventual stock assessment studies.</p> Olfa BEN ABDALLAH Sonia BAKLOUTI Nader BEN HADJ HAMIDA Basma NAFKHA Othman JARBOUI Copyright (c) 2024 Olfa BEN ABDALLAH, Sonia BAKLOUTI, Nader BEN HADJ HAMIDA, Basma NAFKHA, Othman JARBOUI 2024-05-23 2024-05-23 49 21 29 10.80367/instmbulletin.v49.920 Effect of the green macroalgae Chaetomorpha linum liquid extract on the germination, growth and pigments concentration of the chickpea Cicer arietinum L., 1753 <p>Fertilizer production and application are growing excessively with increased agricultural expenses and environmental damage. As alternatives, organic fertilizers issued from natural resources such as seaweed liquid extracts (SLE) are considered as potential plant biostimulant agents. In this study, the impact of the green algae <em>Chaetomorpha linum </em>SLE on seed germination, yield and pigmenting characteristics of the chickpea <em>Cicer arietinum </em>was investigated in laboratory settings and in pots. Several SLE concentrations varying from 1%, 3%, 5%, 8% to 10% were prepared using distilled water.</p> <p>The application of SLE significantly affected all assessed parameters over the control (0%). Use of SLE at the lowest concentration (1%) showed maximum seed germination, root and shoot length and contents of total chlorophyll, chl (a), and chl (b). Accordingly, the results suggest that <em>C. linum </em>SLE could be used as an alternative organic fertilizer because it is environmentally friendly and profitable. This extract, rich in plants growth components, could find its future use in various sectors.</p> Leila CHEBIL AJJABI Leila KTARI Imen BEN DOUAOUA Marwa MELKI Copyright (c) 2024 Leila CHEBIL AJJABI, Leila KTARI, Imen BEN DOUAOUA, Marwa MELKI 2024-06-04 2024-06-04 49 30 43 10.80367/instmbulletin.v49.1684