Depistage du virus <Ostreid herpesvirus>-1 chez l’huitre creuse du pacifique <Crassostrea gigas> collectee dans un élevage conchylicole situe dans la lagune de Bizerte

Authors

  • Yacine BELKHOJDA Ecole nationale de médecine vétérinaire de Sidi Thabet
  • Dorsaf EL AMRI Université de Carthage. Institut National des Sciences et Technologie de la Mer - INSTM
  • Rym KLEIFI Ecole nationale de médecine vétérinaire de Sidi Thabet
  • Ines NJEH Institution de la Recherche et de l'Enseignement Supérieur Agricoles (IRESA). Direction Générale des Services Vétérinaires
  • Zied BOUSLEMA Institut Pasteur Tunis
  • Ines TLIBA Institution de la Recherche et de l'Enseignement Supérieur Agricoles (IRESA), 1Direction Générale des Services Vétérinaire

Keywords:

Ostreid herpesvirus, Crassostrea gigas, Screening, Elevage, Breeding, Hollow oyster, Pacific Oyster, Tunisia, Bizerte Lagoon

Abstract

The infection of the cupped Pacific oyster Crassostrea gigas by the virus Ostreid herpesvirus-1 is a serious disease which may induce catastrophic mortalities. Such phenomena may hinder the development of the oyster industry in Tunisia. This article presents a study whose objective was the detection of this virus, by the techniques of conventional PCR and real time PCR, using specific primer pairs C2/C6 and OsHVDPFor/OsHVDPRev, among 320 Pacific cupped oysters from an oyster farm located in the lagoon of Bizerte. The results obtained by the two techniques were negative for all of the analyzed samples, suggesting that there was no virus circulation within the livestock during the sampling period and that the oysters analyzed were free of herpes virus.

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Published

2016-11-28

How to Cite

1.
BELKHOJDA Y, EL AMRI D, KLEIFI R, NJEH I, BOUSLEMA Z, TLIBA I. Depistage du virus &lt;Ostreid herpesvirus&gt;-1 chez l’huitre creuse du pacifique &lt;Crassostrea gigas&gt; collectee dans un élevage conchylicole situe dans la lagune de Bizerte. Bull. Inst. Natl. Sci. Technol. Mer [Internet]. 2016Nov.28 [cited 2024May20];43:81-5. Available from: https://www.instm-bulletin.tn/index.php/bulletin/article/view/367

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